WEBINAR SERIES—Cardiac Tissue Slices: Preparation, Data Acquisition and Analysis

October 5, 2022
11 a.m. EDT

Cardiac tissue slices preserve native myocardial tissue, including fibroblasts and myocytes, better than isolated cardiomyocytes. Unlike whole heart studies, slices are better suited for acquisition of functional parameters, such as calcium transients. However, cardiac slices have not been widely utilized and their preparation remains a significant challenge for many labs. In this webinar, Bradley Palmer, PhD, assistant professor at the University of Vermont and a research and development engineer at IonOptix, a Massachusetts-based provider of cardiovascular research instruments, shows how to successfully prepare cardiac slices for measurements of force and calcium, work loops, and stress and strain. Palmer will also demonstrate data analysis and interpretation. 

Key topics include: 

• how to successfully prepare cardiac slices, 

• the types of data that can be acquired from cardiac slices, and 

• how to analyze and interpret data from cardiac slices. 

Speaker

Bradley Palmer, PhD, is an assistant professor at the University of Vermont and a research and development engineer at IonOptix, a Massachusetts-based provider of cardiovascular research instruments, His original research focuses on demonstrating the molecular basis of cardiac muscle relaxation function, specifically in the areas of myofilament basis of muscle dynamic stiffness and zinc-dependent alterations in calcium regulation.