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May 30, 2024
11 a.m. EDT
In this webinar, Melanie White, PhD, of the University of Sydney in Australia, will present the various methods for assessing cardiac function in the context of pathology, spanning from in vitro to in vivo techniques. She will also demonstrate how she integrates these techniques with cutting-edge mass spectrometry in her research on cardiovascular disease pathogenesis.
Understanding the dynamics of the heart and how it changes with pathology is a question that can be addressed from many angles. The best approaches must start by selecting an appropriate method to assess cardiac function that permits both real-time assessment and downstream analysis. From in vitro preparations, including isolated cardiomyocytes, stem cells and cardiac organoids, to ex vivo approaches using Langendorff and working heart systems, and finally in vivo, whole system approaches, the approaches are as varied as the outcomes. White will also discuss how she approaches this aspect of her research to align with cutting-edge mass spectrometry.
Key Topics Include:
- method considerations to address different biological questions,
- top tips and tricks for improving success using ex vivo cardiac perfusion, and
- the downstream implications for the various approaches to basic cardiac functional studies.
Speaker
Melanie White, PhD
Heart Foundation Future Leader Fellow, School of Medicine, The University of Sydney
Melanie White, PhD, is the principal investigator of the cardiometabolic proteomics group in the Charles Perkins Society at the University of Sydney in Australia. She has over 20 years of experience in pre-clinical models of cardiovascular disease and cutting-edge mass spectrometry. White’s group research goals include understanding more about how cells adapt to their changing environment by altering proteins using post-translational modifications.