Prenatal Stress May Be behind Tumors, Inflammation in Mice

Prenatal stress found to negatively affect key stem cell receptors

Rockville, Md. (January 19, 2023)—A new mouse study suggests prenatal stress during late gestation can lead to inflammation in adult male offspring and tumor development in adult female offspring, according to researchers at the University of Toulouse in France. Activation of two key receptors in the colon, the protease-activated receptor 2 (PAR2) and muscarinic receptor 3 (M3), triggers these two adverse conditions. The findings are published ahead of print in the American Journal of Physiology-Gastrointestinal and Liver Physiology, and the study has been chosen as an APSselect article for January.

“The deregulation of stem cell proliferation, and therefore of the regeneration of intestinal epithelium, is a high-priority target for therapies in inflammation and cancer pathologies.” —Claire Racaud-Sultan, MD, PhD

In this study, physiologists were trying to understand the roles played by critical regulators of intestinal stem cell functions during stress conditions. Pregnant mice were randomly subjected to stress, such as bright lights, from days 13–18 of gestation. The stress period ran 30 minutes each time three times a day in three-hour intervals. They found stem cells from the colons of male and female mice are regulated differently by PAR2 and M3. In males, PAR2 plays a critical role in the control of intestinal stem cell proliferation, but in females this function is supported by M3.

After birth, the function of stem cells is adversely modified due to prenatal stress, likely triggering serious intestinal conditions such as chronic inflammation and colorectal cancer. In males, the brake exerted by PAR2 on stem cell proliferation could lead to inflammation. In females, a switch of the M3 action could potentially cause abnormal cell growth that causes tumor development.

More studies are needed, but researchers believe the results of this study could be used to improve human health by targeting PAR2 and M3 receptors as an alternative therapy for inflammation and cancer.

“PAR2 and M3 antagonists, already available, might be used to restore the regeneration of the epithelium and its important role as a barrier for pathogens,” said Claire Racaud-Sultan, MD, PhD, corresponding author of the study. “On the other hand, our work shows that colon stem cells from prenatally stressed females present a potent phenotype with the association of high survival and proliferative capacities, which could pave the way for the targeting of PAR2 and M3 to counteract tumor progression.”

Read the full article, “Prenatal stress induces changes in PAR2- and M3-dependent regulation of colon primitive cells.” It is highlighted as one of this month’s “best of the best” as part of the American Physiological Society’s APSselect program. Read all of this month’s selected research articles.

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Physiology is a broad area of scientific inquiry that focuses on how molecules, cells, tissues and organs function in health and disease. The American Physiological Society connects a global, multidisciplinary community of more than 10,000 biomedical scientists and educators as part of its mission to advance scientific discovery, understand life and improve health. The Society drives collaboration and spotlights scientific discoveries through its 16 scholarly journals and programming that support researchers and educators in their work.